Beilstein J. Nanotechnol.2022,13, 1458–1472, doi:10.3762/bjnano.13.120
Southeast Asian box turtle, Cuora amboinensis, is an ecologically important endangered species which needs an onsite monitoring device to protect it from extinction. An electrochemicalDNAbiosensor was developed to detect the C. amboinensis mitochondrial cytochrome b gene based on an in silico designed
in the range of 1 × 10−11–5 × 10−6 M with a limit of detection of 0.85 × 10−12 M. This indicates that the proposed biosensor has the potential to be applied for the detection of real turtle species.
Keywords: box turtle; DNA detection; electrochemicalDNAbiosensor; nanocomposite; screen-printed
ssDNA recognition elements [31]. As per our observation, this is the first application of an electrochemicalDNAbiosensor for BT detection. Kusnin et al. [57] employed silicon nanowires/platinum nanoparticles (SiNWs/PtNPs) on a SPCE for the detection of porcine DNA. The few studies conducted earlier
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Figure 1:
The Cuora amboinensis specific probe (31 bases) and corresponding aligned sequences. A, T, G, C (A,...